Pooled Error Variance for Factorial Effects
When a $2^k$ factorial design is replicated $m$ times, the replicated observations provide a pooled estimate $s^2$ of the experimental error variance.
This leads to
$$ \operatorname{Var}(\text{effect}) = \frac{4 s^2}{m 2^k}. $$That variance is the basis for standard errors and significance tests for factorial effects.